Urinary proteins studied by use of isoelectric focusing. I. Tubular malfunction in association with exposure to cadmium.

Isoelectric focusing in thin-layer polyacrylamide gel has been applied to studies of urinary proteins. We could differentiate the following patterns: tubular, glomerular with high and low selectivity, as well as mixed glomerular and tubular patterns, as seen in some cases of chronic pyelonephritis and uremia. The typical tubular-protein patterns-i.e., as developed in association with cadmium exposure-were characterizedby elevatedexcretion.ff2-microgIobulin. Possibly the severity of renal malfunction can be assessed by the isoelectric focusing technique. To identify the protein zones, we in some cases complemented isoelectric focusing with electrophoresis at right angles in acrylamide gel and also in agarose gel containing antibodies to provide additional information. Additional Keyphrases: diagnostic acid #{149}f32-microglobulin #{149}environmental hazards #{149}renal disease, tubular and glomerular